Open AccessMicrobispora catharanthi sp. nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus
Author(s) -
Nattaporn Klykleung,
Masahiro Yuki,
Takuji Kudo,
Moriya Ohkuma,
Wongsakorn Phongsopitanun,
Pattama Pittayakhajonwut,
Somboon Tanasupawat
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003858
Subject(s) - catharanthus roseus , biology , botany , endophyte
Strain CR1-09 T , an actinomycete isolated from the root of Catharanthus roseus , was taxonomically studied based upon polyphasic approaches. The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia. It contained meso -diaminopimelic acid in cell wall peptidoglycans. The major menaquinones were MK-9 (H 4 ) and MK-9 (H 2 ). The predominant cellular fatty acids were iso-C 16 : 0 and C 16 : 0 . The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids. Strain CR1-09 T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650 T (99.5 %). Based on the polyphasic approach, DNA–DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora , for which the name Microbispora catharanthi is proposed. The type strain is strain CR1-09 T (=JCM 30045 T =TISTR 2273 T ).