Open AccessGudongella oleilytica gen. nov., sp. nov., an aerotorelant bacterium isolated from Shengli oilfield and validation of family Tissierellaceae
Author(s) -
KeJia Wu,
Lirong Dai,
Bo Tu,
Xue Zhao,
Hui Zhang,
Yu Deng,
Paul A. Lawson,
Lei Cheng
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003854
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , bacteria , genus , phylogenetics , gene , microbiology and biotechnology , botany , genetics , anatomy
A novel Gram-stain-positive, rod shaped and anaerobic bacterium, designated as W6 T , was isolated from Shengli oilfield in China. Strain W6 T was observed to grow from 20 to 45 °C with pH 6.5–9.0 (optimally at 40 °C and pH of 7.5) and without addition of NaCl. The major cellular fatty acids were iso-C 15 : 0 (29.1%), C 14 : 0 (27.0%) and C 16 : 0 (12.2%), and the main polar lipids were lipids (L) and aminolipids (AL). The DNA G+C content is 42.9 mol%. Based on 16S rRNA gene sequence analysis, strain W6 T showed highest similarities to Tissierella creatinini DSM 9508 T (94.9%) and Soehngenia saccharolytica DSM 12858 T (94.1%). The morphological, physiological, biochemical, phylogenetic and phylogenomic analyses demonstrated strain W6 T (CCAM 534 T =DSM 28124 T =CGMCC 1.5291 T ) represents a novel species in a new genus , for which the name Gudongella oleilytica gen. nov. sp. nov. is proposed. The family Tissierellaceae is proposed as a new family containing the genera Anaerosalibacter , Gudongella , Keratinibaculum , Soehngenia , Sporanaerobacter , Tepidimicrobium , Tissierella , Urmitella and species Clostridium ultunense based on the phylogenetic and phylogenomic analyses.