Open AccessBacillus caeni sp. nov., isolated from mangrove sediment
Author(s) -
Kunlian Mo,
Huiqin Huang,
Siqi Bao,
Yonghua Hu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003853
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , bacteria , microbiology and biotechnology , mangrove , bacillus (shape) , oxidase test , botany , cell wall , biochemistry , ecology , enzyme , genetics , anatomy
A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195 T , was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China. Cells of the strain were oxidase-negative but positive for catalase and nitrate reduction. Strain HB172195 T was found to grow at 15–50 °C (optimum, 25–40 °C), pH 5.0–9.0 (optimum, pH 7.0) and in 1.0–11.0 % (w/v) NaCl (optimum, 3–6 %). Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso -diaminopimelic acid. The predominant cellular fatty acids were anteiso-C 15 : 0 , anteiso-C 17 : 0 and C 16 : 1 ω 7c alcohol. The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid. The genomic DNA G+C content was 40.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72 T (96.3%), Bacillus algicola KMM 3737 T (96.2 %) and Bacillus haemicentroti JSM 076093 T (95.5 %). Based on polyphasic taxonomic characterization, strain HB172195 T is considered to represent a novel species, for which the name Bacillus caeni sp. nov. is proposed. The type strain is HB172195 T (=CGMCC 1.16730 T =JCM 33411 T ).