Open AccessPseudomonas rhizoryzae sp. nov., isolated from rice
Author(s) -
Xing Wang,
Shanwen He,
Hebao Guo,
Kyu Kyu Thin,
Jieying Gao,
Yao Wang,
Xiaoxia Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003852
Subject(s) - biology , 16s ribosomal rna , pseudomonas , phylogenetic tree , genomic dna , microbiology and biotechnology , phylogenetics , ribosomal rna , homology (biology) , sequence analysis , gene , bacteria , genetics
Two yellow-pigmented, Gram-stain-negative and rod-shaped bacterial strains, designated as RY24 T and ZYY160, were isolated from rice. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strains RY24 T and ZYY160 belonged to the genus Pseudomonas , and the 16S rRNA gene sequence similarity was 100 % The DNA homology between the two strains was 99.7 %. The 16S rRNA and rpoD gene sequences of the two strains showed highest similarity values to Pseudomonas oryzihabitans CGMCC 1.3392 T and Pseudomonas psychrotolerans DSM 15758 T (sharing 99.31 and 94.34 %, respectively). The major fatty acids of two strains were identified as summed feature 8 (C 18:1 ω7 c and/or C 18:1 ω6 c ), C 16;0 and summed feature 3 (C 16:1 ω7 c and/or C 16:1 ω6 c ), and the major respiratory quinone was identified as ubiquinone Q-9, which are typical chemotaxonomic features of members of the genus Pseudomonas . The genomic DNA G+C contents of strains RY24 T and ZYY160 were determined to be 64.25 and 64.21 mol%, respectively. The DNA–DNA relatedness and average nucleotide identity values between the two strains and their closely related type strains were below 36 and 90 %, which supported that RY24 T and ZYY160 represent a novel species in the genus Pseudomonas . Phylogenetic and chemotaxonomic evidence, together with phenotypic characteristics, showed that the two isolates constitute a novel species of the genus Pseudomonas . The type strain is RY24 T (JCM 33201 T =ACCC 61555 T ), for which the name Pseudomonas rhizoryzae sp. nov. is proposed.