Open AccessDevosia ginsengisoli sp. nov., isolated from ginseng cultivation soil
Author(s) -
Xiao-Tian Quan,
Muhammad Hameed Siddiqi,
Qingzhen Liu,
Sangmi Lee,
WanTaek Im
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003843
Subject(s) - biology , ginseng , botany , soil microbiology , traditional medicine , ecology , soil water , medicine , alternative medicine , pathology
A Gram-stain-negative, strictly aerobic, motile, ivory-coloured and rod-shaped bacterium (designated Gsoil 520 T ) isolated from ginseng cultivation soil was characterized by using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 520 T was observed to grow optimally at 30 °C and pH 7.0 on Reasoner's 2A agar medium. The results of phylogenetic analysis, based on 16S rRNA gene sequence similarities, indicated that Gsoil 520 T belongs to the genus Devosia of the family Hyphomicrobiaceae and was most closely related to Devosia epidermidihirudinis E84 T (98.0 %), Devosia yakushimensis Yak96B T (97.7 %), Devosia neptuniae J1 T (97.7 %) and Devosia chinhatensis IPL18 T (96.8 %). The complete genome of strain Gsoil 520 T is a presumptive circular chromosome of 4 480 314 base pairs having G+C content of 63.7 mol%. A total of 4 354 genes, 4 303 CDS and 43 rRNA genes were assigned a putative function. The major isoprenoid quinone was Q-10. The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminolipids (AL1 and AL3). The predominant fatty acids of strain Gsoil 520 T were C 18 : 1 ω7 c 11-methyl, C 16 : 0 and C 18 : 1 ω7 c /C 18 : 1 ω6 c (summed feature 8) supporting the affiliation of strain Gsoil 520 T to the genus Devosia . The low values of DNA–DNA hybridization distinguished strain Gsoil 520 T from the recognized species of the genus Devosia . Thus, the novel isolate represents a novel species of the genus Devosia , for which the name Devosia ginsengisoli sp. nov. is proposed, with the type strain Gsoil 520 T (=KACC 19440 T =LMG 30329 T ).