Open AccessRubrivivax albus sp. nov., isolated from a freshwater pond
Author(s) -
Shih-Yi Sheu,
Zhi-Hao Li,
Chiu-Chung Young,
Wen-Ming Chen
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003829
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , genomic dna , gene , microbiology and biotechnology , strain (injury) , bacteria , phylogenetics , genetics , anatomy
Strain ICH-3 T , isolated from a freshwater pond in Taiwan 9ROC), was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that ICH-3 T is affiliated with the species in the genus Rubrivivax . ICH-3 T was most closely related to Rubrivivax benzoatilyticus JA2 T and Rubrivivax gelatinosus NCIB 8290 T with 97.5 and 97.4 % 16S rRNA gene sequence similarity. The average nucleotide identity and digital DNA–DNA hybridization identity between ICH-3 T and the two closely related strains were 77.3 and 20.9–21.0 %, respectively, indicating that ICH-3 T represents a novel species of the genus Rubrivivax . Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped and formed white colonies. Optimal growth occurred at 30 °C, pH 7.5–8.0 and with 0.5 % NaCl. The major fatty acids (>20 %) of ICH-3 T were summed feature 3 (comprising C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and C 16 : 0 . The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and two uncharacterized phospholipids. The major isoprenoid quinone was Q-8. The genomic DNA G+C content of ICH-3 T was 70.3 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain ICH-3 T should be classified as representing a novel species of the genus Rubrivivax , for which the name Rubrivivax albus sp. nov. is proposed. The type strain is ICH-3 T (=BCRC 81155 T =LMG 30930 T =KCTC 62866 T ).