Open AccessKocuria coralli sp. nov., a novel actinobacterium isolated from coral reef seawater
Author(s) -
Jie Li,
Si Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003825
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , genus , type species , strain (injury) , microbiology and biotechnology , bacteria , botany , gene , genetics , anatomy
An actinobacterial strain, SCSIO 13007 T , was isolated from seawater collected from the Luhuitou fringing reef at a depth of 4.2 m. Phylogenetic and phenotypic properties of the organism supported the hypothesis that it represented a member of the genus Kocuria . Phylogenetic analysis indicated that the levels of 16S rRNA gene sequence similarity between SCSIO 13007 T and type strains of other recognized members of the genus Kocuria were lower than 96.99 %. Growth in the presence of up to 15 % (w/v) NaCl was a distinctive characteristic of SCSIO 13007 T . Other biochemical and physiological properties and the major fatty acids also differentiated the isolate from its phylogenetically closest relative Kocuria subflava YIM 13062 T . The menaquinone types were MK-7(H 2 ) and MK-8(H 2 ). Major cellular fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and anteiso-C 17 : 0 . The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified lipid and an unidentified aminolipid. The DNA G+C content was 73.7 mol%. The combined genotypic and phenotypic data indicated that strain SCSIO 13007 T represents a novel species of the genus Kocuria , for which the name Kocuria coralli sp. nov. is proposed; the type strain is SCSIO 13007 T (=DSM 27811 T =NBRC 109942 T ).