Open AccessCroceicoccus sediminis sp. nov., isolated from coastal sediment
Author(s) -
Zhongdong Huang,
Aoqiang Zhang,
Qiliang Lai,
Jianjun Yuan
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003823
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , monophyly , ribosomal rna , botany , gene , genetics , clade , anatomy
A Gram-stain-negative, cocci or short rod-shaped, yellow-pigmented bacterium, designated strain S2-4-2 T , was isolated from coastal sediment of Spartina alterniflora in Quanzhou Bay, PR China. Growth was observed at 15–40 °C (optimum, 30 °C) and pH 6.0–8.0 (optimum, pH 7.0). Strain S2-4-2 T tolerated 0–10 % NaCl (optimum, 1 %). The 16S rRNA gene of strain S2-4-2 T showed highest sequence similarity to Croceicoccus pelagius Ery9 T (98.2 %), followed by Croceicoccus naphthovorans PQ-2 T , Croceicoccus marinus E4A9 T and Croceicoccus mobilis Ery22 T (97.6%, 96.3 and 96.3 %, respectively). Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on 92 up-to-date bacterial core gene sets indicated that strain S2-4-2 T forms a distinct monophyletic branch affiliated to the genus Croceicoccus . The average nucleotide identity value between strain S2-4-2 T and its close relatives were estimated to be 74.8–85.7 %. The respiratory quinone was found to be Q-10. The predominant fatty acids (>10 %) were identified as summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) and summed feature 3 (C 16 : 1 ω7 c and C 16 : 1 ω6 c ). The polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and an unidentified phospholipid. The draft genome size of strain S2-4-2 T was 3.5 Mb with a genomic G+C content of 63.0 mol%. Based on these results, strain S2-4-2 T is concluded to represent a novel species within the genus Croceicoccus , for which the name Croceicoccus sediminis sp. nov. is proposed with the type strain S2-4-2 T (=MCCC 1K03706 T =KCTC 72146 T ).