Open AccessAsticcacaulis tiandongensis sp. nov., a new member of the genus Asticcacaulis, isolated from a cave soil sample
Author(s) -
Xing Kui Zhou,
Ying Huang,
Miao Li,
Xiaofei Zhang,
Yu-Qian Wei,
Shi-Chun Qin,
Ti Kun Zhang,
Xuejian Wang,
Jian Jin Liu,
Liang Wang,
Ziyi Liu,
Ming He Mo
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003818
Subject(s) - biology , cave , genus , zoology , botany , ecology
A Gram-stain negative, aerobic, motile and rod-shaped bacterium, designated strain 3.1105 T , was isolated from a karst district soil sample collected from Tiandong cave, Guizhou province, south-west PR China. The isolate grew at 10-40 °C and pH 5.0-8.0 and tolerated up to 1 % NaCl (w/v) on R2A medium, with optimal growth at 25-30 °C, pH 7.0 and 0 % NaCl (w/v). Cells showed oxidase-positive and catalase-positive reactions. The respiratory quinone was Q-10. The predominant cellular fatty acids contained C 18 : 1 ω7 c 11-methyl, summed feature 8 (C 18 : 1 ω7 c or C 18 : 1 ω6 c ), C 16 : 0 and C 17 : 0 . The major polar lipids were phosphatidylglycerol and monoglycosyldiglycerides. The genomic DNA G+C content was 56.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that 3.1105 T should be affiliated to the genus Asticcacaulis and showed highest 16S rRNA gene sequence similarity values with Asticcacaulis excentricus CB 48 T (96.0 %), Asticcacaulis endophyticus ZFGT-14 T (95.3 %) and lower than 95.3 % similarity to other species of the genus Asticcacaulis . The polyphasic taxonomic characteristics indicated that strain 3.1105 T represents a novel species of the genus Asticcacaulis , for which the name Asticcacaulis tiandongensis sp. nov., (type strain 3.1105 T =KCTC 62978 T =CCTCC AB 2018268 T ) is proposed.