Open AccessNioella ostreopsis sp. nov., isolated from toxic dinoflagellate, Ostreopsis lenticularis
Author(s) -
Yingjie Qiao,
Zhiwei Jiang,
Xin Zhou,
Ruonan Zhang,
Yiru Wu,
Linghao Lou,
Zhuang Ma,
Dongfang Wang,
Yaming Ge,
Xiaoling Zhang,
Xinwei Yu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003816
Subject(s) - biology , 16s ribosomal rna , dinoflagellate , phylogenetic tree , microbiology and biotechnology , gene , bacteria , biochemistry , botany , genetics
A novel short-rod-shaped bacterial strain with poly-β-hydroxybutyric acid granules inside, designated as Z7-4 T , was isolated from a culture of a marine dinoflagellate with palytoxin-producing capacity, Ostreopsis lenticularis OS06, collected from the East China Sea. Cells of Z7-4 T were Gram-stain-negative, non-motile, strictly aerobic, 0.9–1.2 µm wide and 2.0–3.9 µm long. Growth occurred in 1–4 % (w/v) NaCl, at 15–37 °C and at pH 5.0–10.0, with optimum growth in 3.5 % (w/v) NaCl, at 30 °C and at pH 7.0. Analysis of 16S rRNA gene sequence revealed that Z7-4 T shared the highest 16S rRNA gene sequence similarities with Nioella aestuarii JCM 30752 T (98.8 %), followed by Nioella sediminis KCTC 42144 T (98.6 %) and Nioella nitratireducens KCTC 32417 T (96.9 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that Z7-4 T clearly represented a member of the genus Nioella within the family Rhodobacteraceae . The respiratory quinone of Z7-4 T was identified as Q-10. Polar lipids of Z7-4 T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. The major fatty acids were summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) and C 16 : 0 . The DNA G+C content of Z7-4 T was 63.0 mol%. DNA–DNA hybridization values of the isolate against the closely related type strains were far below the 70 % limit for species delineation. The average nucleotide identity and in silico DNA–DNA genome hybridization relatedness between Z7-4 T and the closely related members of the genus Nioella , N. sediminis KCTC 42144 T and N. nitratireducens KCTC 32417 T , were 91.1 and 46.3 %, and 79.3 and 19.4 %, respectively. On the basis of the results of polyphasic analysis, Z7-4 T is proposed to represent a novel species of the genus Nioella , for which the name Nioella ostreopsis sp. nov. is proposed. The type strain of Nioella ostreopsis is Z7-4 T (=KCTC 62459 T =CCTCC AB 2017231 T )