Open AccessPaenibacillus piri sp. nov., isolated from urban soil
Author(s) -
Ngoc Hoang Trinh,
Jaisoo Kim
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003811
Subject(s) - biology , 16s ribosomal rna , paenibacillus , strain (injury) , microbiology and biotechnology , bacteria , agar , nutrient agar , agar plate , phosphatidylglycerol , genetics , anatomy , phospholipid , membrane , phosphatidylcholine
A rod-shaped, Gram-stain-positive bacterial strain, MS74 T , was isolated from soil beside Itaewon road, Seoul, Republic of Korea. The strain could grow well on R2A, nutrient agar and tryptone soya agar, but not in LB agar. MS74 T tolerated 3.0 % NaCl (w/v), pH 9.0 and a temperature range from 10 to 35 °C (optimal temperature, 28 °C). From the comparison of 16S rRNA gene sequence, the strain is most closely related to Paenibacillus vulneris CCUG 53270 T , Paenibacillus esterisolvens CFH S0170 T , Paenibacillus rigui JCM 16352 T and Paenibacillus yunnanensis YN2 T with similarity percentages of 96.6 %, 96.4 %, 95.9 % and 95.8 % respectively. Major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). The major menaquinone was MK-7. The fatty acids profile mainly consisted of C 15 : 0 anteiso, C 15 : 0 iso, C 16 : 0 iso, and C 16 : 0 . The DNA G+C content of the isolated strain determined from the whole-genome sequence was 51 mol%. MS74 T had an average nucleotide identity (ANI) value of 73.9 % and a digital DNA-DNA hybridization (dDDH) value of 20.6 % with most closely related strain, Paenibacillus vulneris CCUG 53270 T . On the basis of phenotypic, chemotypic and genotypic evidence, the isolate was identified as representing a novel species of the genus Paenibacillus , for which the name Paenibacillus piri sp. nov. is proposed. The type strain is MS74 T (=KACC 19385 T =DSM 105496 T ).