Open AccessPutridiphycobacter roseus gen. nov., sp. nov., isolated from Antarctic rotten seaweed
Author(s) -
Xiujuan Wang,
Lin Xu,
Ning Wang,
He-Min Sun,
Xiu-Lan Chen,
Yuzhong Zhang,
Mei Shi,
Xiying Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003809
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , genus , phylogenetics , botany , bacteria , genomic dna , lineage (genetic) , gene , genetics , anatomy
A Gram-stain-negative, pink-pigmented, rod-shaped, non-flagellated, aerobic bacterium, designated strain SM1701 T , was isolated from a rotten seaweed collected off Fildes Peninsula, King George Island, West Antarctica. The strain grew at 4-30 °C, pH 6.0-8.0 and with 0.5-5 % (w/v) NaCl. It hydrolysed gelatin and Tweens (40, 60 and 80), but did not reduce nitrates to nitrites. The major cellular fatty acids of strain SM1701 T were iso-C 15 : 0 , iso-C 15 : 1 G, iso-C 16 : 1 G, C 16 : 0 and iso-C 17 : 0 3-OH. Polar lipids included phosphatidylethanolamine, one unidentified aminolipid, two unidentified glycolipids and one unidentified aminoglycolipid. The major respiratory quinone was MK-7. The genomic DNA G+C content of strain SM1701 T was 34.1 mol%. It showed high 16S rRNA gene sequence similarities to Crocinitomix algicola (93.8 %) and Crocinitomix catalasitica (92.5 %) and less than 91 % sequence similarities to other known members in the family Crocinitomicaceae . Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1701 T constituted a distinct lineage within the family Crocinitomicaceae . The phylogenetic trees based on concatenated 261 protein sequences from genome sequences showed that strain SM1701 T occupied a branch separated from those of known genera in the family of Crocinitomicaceae , indicating it may belong to a new genus. On the basis of the polyphasic characterization of strain SM1701 T in this study, it is considered to represent a novel species in a new genus in the family Crocinitomicaceae , for which the name Putridiphycobacter roseus gen. nov., sp. nov. is proposed. The type strain is SM1701 T (=KCTC 62302 T =NBRC 113201 T =CGMCC 1.16510 T ).