Open AccessChryseobacterium binzhouense sp. nov., isolated from activated sludge
Author(s) -
Dong Meng,
Yuling Liu,
Rui-Rui Li,
Pengfei Gu,
Xiangyu Fan,
Zida Huang,
ZongJun Du,
Qiang Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003800
Subject(s) - biology , activated sludge , microbiology and biotechnology , chryseobacterium , bacteria , wastewater , waste management , genetics , 16s ribosomal rna , engineering
A golden-pigmented, Gram-strain-negative, aerobic, rod-shaped, non-flagellated and non-gliding bacterium, designated strain lm2 T , was isolated from activated sludge obtained from a wastewater treatment plant in Binzhou (Shandong province, PR China). Growth occurred at 15-45°C (optimum, 30 °C), in the presence of 0-5.0 % (w/v) NaCl (optimum, 0-2.0 %) and at pH 6.5-8.0 (optimum, pH 7.0-7.5). The chemotaxonomic, phenotypic and genomic traits were investigated. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain lm2 T belonged to the genus Chryseobacterium , with highest sequence similarity to Chryseobacterium echinoideorum CC-CZW010 T (97.1 %). Genome sequencing revealed a genome size of 3 611 894 bp and a G+C content of 34.9 mol%. The average nucleotide identity value and the digital DNA-DNA hybridization (dDDH) value between strain lm2 T and C. echinoideorum JCM 30470 T were 87.8 and 34.7 %, respectively. The major respiratory quinone was Menaquinone-6 (MK-6). The major fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and iso-C 17 : 1 ω 9 c and its polar lipids consisted of phosphatidylethanolamine (PE), unidentified lipids (L1-5) and unidentified aminolipids (AL1-4). On the basis of these data, strain lm2 T is considered to represent a novel species of the genus Chryseobacterium , for which the name Chryseobacterium binzhouense sp. nov. is proposed. The type strain is lm2 T (=KCTC 72529 T =CCTCC AB2019126 T ).