Open AccessSolirhodobacter olei gen. nov., sp. nov., a nonphotosynthetic bacterium isolated from oil-contaminated soil
Author(s) -
Cuiwei Chu,
Bin Liu,
Ziyi Lian,
Hongfei Zheng,
Can Chen,
Zonghao Yue,
Lili Li,
Zhongke Sun
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003795
Subject(s) - biology , bacteria , contamination , botany , microbiology and biotechnology , ecology , genetics
A nonphotosynthetic, Gram-stain-negative, rod-shaped and motile strain, designated Pet-1 T , was isolated from oil-contaminated soil collected from Daqing oil field in China. Optimal growth occurred at 37 °C, pH 5.5 and in 1 % (w/v) NaCl. Q-10 was the sole respiratory quinone. The most abundant fatty acid was C 18 : 1 ɷ7 c /C 18 : 1 ɷ6 c (67.4 %). The major polar lipids were phosphatidylglycerol, aminolipid, phosphatidylethanolaine, phosphatidycholine, two unidentified lipids and two unidentified phospholipids. The genomic DNA G+C content was 69.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that Pet-1 T shared the highest similarity (95.1 %) to Rhodobacter vinaykumarii DSM 18714 T , followed by Sinorhodobacter populi sk2b1 T (95.0 %) and Haematobacter massiliensis CCUG 47968 T (95.0 %). In the phylogenetic tree, strain Pet-1 T formed a separate branch from the closely related genera Rhodobacter, Pararhodobacter, Defluviimonas and Rhodovulum within the family Rhodobacteraceae . Based on the data from the current polyphasic study, it is proposed that the isolate is a novel species of a novel genus within the family Rhodobacteraceae , with the name Solirhodobacter olei gen. nov., sp. nov. The type strain of the type species is Pet-1 T (=KCTC 72074 T =CCTCC AB 2018368 T ).