Algoriphagus aquimaris sp. nov., isolated from seashore sand

Strain F21 T , a marine, aerobic, Gram-negative, rod-shaped bacterium, was isolated from seashore sand sampled in Pohang, Republic of Korea. Cells of strain F21 T were non-motile, catalase-positive, oxidase-positive, non-spore-forming and formed pinkish-red colonies on marine agar. The strain grew optimally at 37°C, pH 7 and in the presence of 2–3 % NaCl (w/v). Analysis of the 16S rRNA gene sequence of strain F21 T revealed that it belonged to the genus Algoriphagus , family Cyclobacteriaceae , with similarity values of 98.1 and 96.8 % to Algoriphagus marincola DSM 16067 T and Algoriphagus ornithinivorans IMSNU 14014 T , respectively. When comparing the genome sequence of F21 T with those of the type strains of six species of the genus Algoriphagus , the values obtained were below the thresholds for analyses of average nucleotide identity (71.8–92.7 %) and in silico DNA–DNA hybridization using the Genome-to-Genome Distance Calculator (14.7–75.2 %). The DNA G+C content of strain F21 T was 42.0 mol%. The chemotaxonomic characteristics of F21 T included MK-7 as the predominant isoprenoid quinone, iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c ) as major cellular fatty acids, and phosphatidylcholine and phosphatidylethanolamine as major polar lipids. On the basis of phenotypic and chemotaxonomic properties, phylogenetic distinctiveness, and genomic data, we named strain F21 T as Algoriphagus aquimaris sp. nov. and proposed that strain F21 T (=KEMB 2250–007 T = KCTC 72106 T =JCM 33187 T ) in the genus Algoriphagus represents a novel species.