Open AccessAzospirillum thermophilum sp. nov., isolated from a hot spring
Author(s) -
Zhuo-Li Zhao,
Ming Hong,
Chenlong Ding,
Wei-Li Ji,
Li-Jiao Cheng,
Mingming Niu,
Yanmin Zhang,
Lingyu Zhang,
Xiaolin Meng,
Guoxing Nie
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003788
Subject(s) - biology , 16s ribosomal rna , microbial inoculant , microbiology and biotechnology , strain (injury) , phosphatidylglycerol , bacteria , genomic dna , botany , gene , phospholipid , phosphatidylcholine , biochemistry , genetics , membrane , anatomy
A novel Gram-staining negative, aerobic, motile by flagellum, rod-shaped bacterium, designated CFH 70021 T was isolated from a hot spring soil sample collected from Tengchong, Yunnan province, PR China. Growth of CFH 70021 T occurred at 15-50 °C (optimum 50 °C), pH 5.0-7.0 (optimum pH 7.0) and with 0-3.0 % (w/v) NaCl (optimum 0 %, w/v). The genome of CFH 70021 T consisted of four complete circular chromosomes and five plasmids, the genomic DNA G+C content was 69.3 mol%. Comparison of the 16S rRNA gene sequences indicated that CFH 70021 T represented a member of the genus Azospirillum and showed close relationship with the type strains of Azospirillum agricola CC-HIH038 T (97.8 %), Azospirillum rugosum IMMIB AFH-6 T (97.6 %), Azospirillum doebereinerae GSF71 T (97.6 %), Azospirillum thiophilum DSM 21654 T (97.4 %) and Azospirillum picis IMMIB TAR-3 T (97.2 %). The polar lipids of CFH 70021 T contained diphosphatidylglycerol, phosphatidylmehtylethanolamine, phosphatidylglycerol, phosphatidylcholine, two aminolipids and an unidentified phospholipid. The predominant cellular fatty acids (>10 %) included C 19:0 cyclo ω 8c (11.4 %), C 16 : 0 (27.6 %) and summed feature 8 (C 18:1 ω7 c /C 18:1 ω6 c , 40.9 %). The major isoprenoid quinone was Q-10. On the basis of the low ANIb result (<78 %) and different phenotypic and chemotaxonomic characters, we conclude that strain CFH 70021 T represents a novel member of the genus Azospirillum , for which the name Azospirillum thermophilum sp. nov. is proposed. The type strain is CFH 70021 T (=KCTC 62259 T = CCTCC AB2018121 T ).