Open AccessAchromobacter veterisilvae sp. nov., from a mixed hydrogen-oxidizing bacteria enrichment reactor for microbial protein production
Author(s) -
Charles Dumolin,
Charlotte Peeters,
Elham Ehsani,
Guillaume Tahon,
Evelien De Canck,
Margo Cnockaert,
Nico Boon,
Peter Vandamme
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003786
Subject(s) - achromobacter , biology , 16s ribosomal rna , achromobacter xylosoxidans , microbiology and biotechnology , strain (injury) , phylogenetic tree , bacteria , sequence analysis , gene , pseudomonas , genetics , anatomy
Strain LMG 30378 T was isolated from a hydrogen-oxidizing bacteria enrichment reactor inoculated with forest soil. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this strain belonged to the genus Achromobacter . Multilocus sequence analysis combined with sequence analysis of a 765 bp nrd A gene fragment both showed Achromobacter agilis LMG 3411 T and Achromobacter denitrificans LMG 1231 T to be the closest-related neighbours to strain LMG 30378 T . Genome sequence analysis revealed a draft genome of 6.81 Mb with a G+C content of 67.2 mol%. In silico DNA-DNA hybridization with A. denitrificans LMG 1231 T and A. agilis LMG 3411 T showed 42.7 and 42.5% similarity, respectively, confirming that strain LMG 30378 T represented a novel Achromobacter species. Phenotypic and metabolic characterization revealed acid phosphatase activity and the absence of phosphoamidase activity as distinctive features. The draft genome composes all necessary metabolic components to fix carbon dioxide and to oxidize molecular hydrogen, suggesting that strain LMG 30378 T is a key organism in the enrichment reactor. Together, these data demonstrate that strain LMG 30378 T represents a novel species of the genus Achromobacter , for which the name Achromobacter veterisilvae sp. nov. is proposed. The type strain is LMG 30378 T (=CCUG 71558 T ).