Open AccessArenimonas terrae sp. nov., isolated from orchard soil
Author(s) -
Dong Min Han,
Byung Hee Chun,
Hyung Min Kim,
Shehzad Abid Khan,
Che Ok Jeon
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003785
Subject(s) - biology , orchard , botany , horticulture
A Gram-stain-negative, strictly aerobic bacterial strain, designated R29 T , was isolated from orchard soil in Hwasun, South Korea. Cells were non-motile rods with catalase and oxidase activities. Growth was observed at 15-40 °C (optimum, 30 °C) and pH 7.0-9.0. Ubiquinone-8 was identified as the predominant isoprenoid quinone. Major polar lipids consisted of phosphatidylethanolamine, six phospholipids, an unidentified aminolipid and two unidentified polar lipids. Strain R29 T contained iso-C 15 : 0 , iso-C 16 : 0 and summed feature 9 (comprising iso-C 17 : 1 ω 9 c /10-methyl-C 16 : 0 ) as major cellular fatty acids. The G+C content of the genomic DNA calculated from the whole-genome sequence was 69.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that R29 T was most closely related to Arenimonas daejeonensis T7-07 T and Arenimonas malthae CC-JY-1 T with sequence similarities of 98.0 and 96.9 %, respectively The average nucleotide identity and in silico DNA-DNA hybridization values between R29 T and the type strains of A. daejeonensis and A. malthae were 87.0 % and 31.6 % and 83.4 % and 26.1 %, respectively. On the basis of the phenotypic, chemotaxonomic and molecular features, strain R29 T represents a novel species of the genus Arenimonas , for which the name Arenimonas terrae sp. nov. is proposed. The type strain is R29 T (=KACC 19896 T =JCM 33216 T ).