Open AccessAlistipes communis sp. nov., Alistipes dispar sp. nov. and Alistipes onderdonkii subsp. vulgaris subsp. nov., isolated from human faeces, and creation of Alistipes onderdonkii subsp. onderdonkii subsp. nov.
Author(s) -
Mitsuo Sakamoto,
Nao Ikeyama,
Yusuke Ogata,
Wataru Suda,
Takao Iino,
Masahira Hattori,
Moriya Ohkuma
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003778
Subject(s) - biology , microbiology and biotechnology , 16s ribosomal rna , bacteria , genetics
Three groups of Gram-stain-negative, obligately anaerobic, rod or coccoid-shaped bacteria, which were phylogenetically assigned in the genus Alistipes belonging to the family Rikenellaceae in the phylum Bacteroidetes , were isolated from the faecal samples of healthy Japanese humans. Group I (strains 5CBH24 T and 6CPBBH3) showed highest 16S rRNA gene sequence similarity to ' Alistipes obesi ' ph8 T (99.73 %). Group II (strain 5CPEGH6 T ) was related to Alistipes shahii WAL 8301 T (96.82 %). Ten strains of group III (3BBH6 T , 5CPYCFAH4, 5NYCFAH2 and others) were related to Alistipes onderdonkii DSM 19147 T (98.96 %). Group I could be differentiated from other strains by the ability to hydrolyse aesculin and the lack of catalase activity. Strain 5CPEGH6 T could be differentiated from A. shahii JCM 16773 T by the inability to hydrolyse aesculin and the lack of catalase activity, and so on. Phenotypic characteristics of group III were similar to those of A. onderdonkii JCM 16771 T . Strains 5CBH24 T , 6CPBBH3 and ' A. obesi ' ph8 T shared 98.8-98.9 % average nucleotide identity (ANI) with each other. In addition, the in silico DNA-DNA hybridization (DDH) values among three strains were 86.7-89.4 %. Strain 5CPEGH6 T showed relatively low values (≤ 84.4 % for ANI ; ≤26.2 % for DDH) with other strains. Three strains in the group III (3BBH6 T , 5CPYCFAH4 and 5NYCFAH2) shared 97.9-99.9% ANI with each other. These three strains showed 96.9-97.3 % ANI with A. onderdonkii DSM 19147 T . The DDH values of strains 3BBH6 T , 5CPYCFAH4 and 5NYCFAH2 among themselves were 80.5-99.8 %, while those compared to A. onderdonkii DSM 19147 T were 71.0-73.4 %. On the basis of the collected data, three novel species, Alistipes communis sp. nov. (5CBH24 T =JCM 32850 T =DSM 108979 T ), Alistipes dispar sp. nov. (5CPEGH6 T =JCM 32848 T =DSM 108978 T ) and Alistipes onderdonkii subsp. vulgaris subsp. nov. (3BBH6 T =JCM 32839 T =DSM 108977 T ), are proposed.