Streptomyces oryziradicis sp. nov., a novel actinomycete isolated from rhizosphere soil of rice (Oryza sativa L.)

A novel actinobacterium, designated strain NEAU-C40 T , was isolated from the rhizosphere soil of rice ( Oryza sativa L.) collected from Northeast Agricultural University in Harbin, Heilongjiang province, north-east PR China, and was characterized using a polyphasic approach. On the basis of results of 16S rRNA gene sequence analysis, strain NEAU-C40 T belongs to the genus Streptomyces , and shares highest sequence similarities with Streptomyces polygonati CGMCC 4.7237 T (97.9%) and Streptomyces abietis DSM 42080 T (97.9%). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Streptomyces . Cell walls contained ll-diaminopimelic acid and the whole-cell hydrolysates were glucose, rhamnose and ribose. The major menaquinones were identified as MK-9(H 8 ) and MK-9(H 6 ). The major fatty acids were iso-C 16:0 , anteiso-C 15:0 , C 16:0 and anteiso-C 17:0 . The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified lipid. The genomic DNA G+C content of strain NEAU-C40 T was 71.8 mol%. Moreover, multilocus sequence analysis based on five other housekeeping genes ( atp D , gyr B , rpo B , rec A and rp B ) and the low level of DNA-DNA relatedness allowed the isolate to be differentiated from its most closely related strains. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-C40 T can be characterized to represent a novel species of the genus Streptomyces , for which the name Streptomyces oryziradicis sp. nov. is proposed. The type strain is NEAU-C40 T (=DSM 107943 T =CCTCC AA 2018038 T ).