Open AccessMarinobacter vulgaris sp. nov., a moderately halophilic bacterium isolated from a marine solar saltern
Author(s) -
Yu Zhang,
Xianchun Zhong,
Wei Xu,
Dan Lü,
Jinxin Zhao,
ZongJun Du
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003774
Subject(s) - biology , 16s ribosomal rna , halophile , phylogenetic tree , microbiology and biotechnology , bacteria , sequence analysis , botany , gene , biochemistry , genetics
A facultatively anaerobic, Gram-stain-negative and non-gliding bacterium, designated F01 T , was isolated from marine solar saltern in Weihai, PR China. Cells of F01 T were 0.2-0.4 µm wide and 1.4-4.1 µm long, weakly catalase-positive and oxidase-negative. Growth of F01 T was determined to occur at 4-40 °C (optimum, 33-37 °C), pH 6.5-8.5 (optimum, 7.0-8.0), and with 0.5-18.0 % (w/v) NaCl (optimum, 3.0-6.0 %). The 16S rRNA gene sequence analysis indicated that F01 T represented a member of the genus Marinobacter within the family Alteromonadaceae . Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was most closely related to Marinobacter algicola DSM 16394 T , with a sequence similarity of 97.5 %. The DNA G+C content of the isolate was 57.6 mol%. The major respiratory quinone of F01 T was ubiquinone-9 (Q-9) and the major fatty acids were anteiso-C 15 : 0 , C 16 : 0 and C 18 : 1 ω9 c . The major polar lipids were phosphoaminolipid, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the results of the phylogenetic analysis and phenotypic properties, it is concluded that F01 T can be considered to represent a novel species in the genus Marinobacter , for which the name Marinobacter vulgaris sp. nov. is proposed. The type strain is F01 T (=MCCC 1H00290 T =KCTC 52700 T ).