Marisediminitalea mangrovi gen. nov., sp. nov., isolated from marine mangrove sediment, and reclassification of Aestuariibacter aggregatus as Marisediminitalea aggregata comb. nov.

Strain GS-14 T was isolated from a mangrove sediment sample collected at Beilun Estuary National Nature Reserve, Guangxi Province, PR China. Cells were Gram-stain-negative, strictly aerobic and rod-shaped with a polar flagellum. Optimal growth occurred in the presence of 3-6 % (w/v) NaCl, at pH 6-8 and at a temperature of 37 °C. The predominant polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone 8 (Q-8) was the sole respiratory quinone. The major fatty acids (>10 % of the total fatty acids) were summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and C 16 : 0 . The DNA G+C content was 47.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GS-14 T had the highest sequence similarity to Aestuariibacter aggregatus WH169 T (96.63 %), Aliiglaciecola coringensis AK49 T (96.56 %) and Alteromonas lipolytica JW12 T (96.22 %). In addition, the OrthoANIu value and dDDH values calculated from the genomes of strain GS-14 T and A. aggregatus WH169 T were 79.5 and 21.9 %, respectively. Based on the polyphasic taxonomic results, strain GS-14 T is considered to represent a novel species in a new genus, for which the name Marisediminitalea mangrovi gen. nov., sp. nov. is proposed. The type strain of Marisediminitalea mangrovi is GS-14 T (=KCTC 72401 T =MCCC 1K03622 T ). Because Aestuariibacter aggregatus WH169 T clustered with strain GS-14 T in the phylogenetic trees and was clearly separated from the two species within the genus Aestuariibacter , it is reclassified as a member of the genus Marisediminitalea as Marisediminitalea aggregata comb. nov. (type strain WH169 T =CGMCC 1.8995 T =LMG 25283 T ). The type species of the genus Marisediminitalea is Marisediminitalea aggregata gen. nov., comb. nov.