Open AccessGryllotalpicola protaetiae sp. nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli
Author(s) -
Hayoung Cho,
Jun Heo,
Moriyuki Hamada,
Tomohiko Tamura,
Satomi Saitou,
Jeong-Seon Kim,
SeungBeom Hong,
Soojin Kim,
Soon-Wo Kwon
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003767
Subject(s) - biology , 16s ribosomal rna , diamino acid , strain (injury) , phylogenetic tree , phosphatidylglycerol , fatty acid , microbiology and biotechnology , biochemistry , gene , phospholipid , gene sequence , anatomy , phosphatidylcholine , membrane
A bacterial strain, designated 2DFW10M-5 T , was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea. Cells of the strain were Gram-stain-positive, non-motile and rod-shaped. The strain grew at the range of 10–37 °C (optimum, 28–30 °C) and pH 4.0–8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium. It was catalase-positive and oxidase-negative. The 16S rRNA gene sequence of strain 2DFW10M-5 T showed the highest similarity to Gryllotalpicola daejeonensis RU-04 T (98.4 %), Gryllotalpicola soli KIS12-7 T (98.2 %), Gryllotalpicola kribbensis PU-02 T (97.5 %), Gryllotalpicola koreensis RU-16 T (97.4 %) and Gryllotalpicola reticulitermitis TS-56 T (97.2 %). The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5 T fell into the radius of the genus Gryllotalpicola . The predominant fatty acid was ω-cyclohexyl-C 17:0 . The polar lipids of strain 2DFW10M-5 T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids. The detected isoprenoid quinones were MK-11 (61.0 %), MK-10 (33.7 %) and MK-12 (5.3 %). The peptidoglycan contained d - and l -alanine, d -glutamic acid, glycine, l -serine and d -lysine with l -lysine as the diamino acid. The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5 T was 69.2 mol%. On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5 T represents a novel species of the genus Gryllotalpicola , for which the name Gryllotalpicola protaetiae sp. nov. is proposed. The type strain is 2DFW10M-5 T (=KACC 19316 T =NBRC 113049 T ).