Open AccessGeorhizobium profundi gen. nov., sp. nov., a piezotolerant bacterium isolated from a deep-sea sediment sample of the New Britain Trench
Author(s) -
Junwei Cao,
Yuli Wei,
Qiliang Lai,
Yunjie Wu,
Junhao Deng,
JianYang Li,
Rulong Liu,
Li Wang,
Jiasong Fang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003766
Subject(s) - biology , trench , sediment , deep sea , bacteria , sample (material) , ecology , oceanography , fishery , paleontology , chemistry , organic chemistry , layer (electronics) , chromatography , geology
A novel alphaproteobacterium, strain WS11 T , was isolated from a deep-sea sediment sample collected from the New Britain Trench. The full-length 16S rRNA gene of strain WS11 T had the highest sequence similarity of 97.6 % to Rhizobium subbaraonis JC85 T , followed by Mycoplana ramosa DSM 7292 T (96.9 %) and Rhizobium azooxidifex Po 20/26 T (96.8 %). Phylogenetic analysis of concatenated 16S rRNA, atpD and recA gene sequences showed that strain WS11 T was deeply separated from the species within the family Rhizobiaceae . Phylogenomic analysis based on the whole-genome protein sequences showed that strain WS11 T formed an independent monophyletic branch in the family Rhizobiaceae , paralleled with the species in the families Brucellaceae and Phyllobacteriaceae within the order Rhizobial es. Cells were Gram-stain-negative, oxidase- and catalase-positive, and aerobic short rods (1.5-2.4×0.9-1.0 µm). Growth was observed at salinities ranging from 0 to 5% (optimum, 1 %), from pH 6.5 to 9 (optimum, pH 7) and at temperatures between 20 and 30 °C (optimum, 28 °C). Strain WS11 T was piezotolerant, growing optimally at 0.1 MPa (range 0.1-70 MPa). The main fatty acid was summed feature 8 (C 18 : 1 ω 7 c /C 18 : 1 ω 6c ). The sole respiratory quinone was ubiquinone-10 (Q-10). The predominant polar lipids were phosphatidylcholine, two unidentified aminophospholipids and an unidentified phospholipid. The genome size was about 4.36 Mbp and the G+C content was 62.3 mol%. The combined genotypic and phenotypic data show that strain WS11 T represents a novel species of a novel genus in the family Rhizobiaceae , for which the name Georhizobium profundi gen. nov., sp. nov. is proposed (type strain WS11 T =MCCC 1K03498 T =KCTC 62439 T ).