Open AccessAliifodinibius saliphilus sp. nov., a moderately halophilic bacterium isolated from sediment of a crystallizing pond of a saltern
Author(s) -
Geon-Yeong Cho,
Kyung-Sook Whang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003765
Subject(s) - halophile , biology , 16s ribosomal rna , phylogenetic tree , bacteria , microbiology and biotechnology , glycolipid , botany , strain (injury) , phylogenetics , genomic dna , terpenoid , gene , biochemistry , genetics , anatomy
Two Gram-stain-negative, moderately halophilic bacteria, designated strains ECH52 T and KHM46, were isolated from the sediment of a grey saltern located in Sinui island at Shinan, Korea. The isolates were aerobic, non-motile, short rods and grew at 15-45 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum, pH 8.0) and with 3-25 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains ECH52 T and KHM46 belonged to the genus Aliifodinibius in the family Balneolaceae with sequence similarities of 94.3-98.6 % and showed the highest sequence similarity to Aliifodinibius halophilus 2W32 T (98.6 %), A. sediminis YIM J21 T (94.7%), A. salicampi KHM44 T (94.6 %) and A. roseus YIM D15 T (94.3 %). The DNA G+C content of the genomic DNA of strain ECH52 T was 40.8 mol%. The predominant isoprenoid quinone was menaquinone-7 (MK-7) and the major cellular fatty acids were iso-C 17 : 1 ω9 c , iso-C 15 : 0 , and C 16 : 1 ω7 c and/or iso-C 15 : 0 2-OH. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified glycolipids and four unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic data, strains ECH52 T and KHM46 are considered to represent a novel species of the genus Aliifodinibius , for which the name Aliifodinibius saliphilus sp. nov. is proposed. The type strain is ECH52 T (=KACC 19126 T =NBRC 112664 T ).