Open AccessAfifella aestuarii sp. nov., a phototrophic bacterium
Author(s) -
Sailaja Buddhi,
G. Suresh,
Deepshikha Gupta,
Ch. Sasikala,
V. Venkata Ramana
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003756
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phototroph , bacteria , microbiology and biotechnology , oxidase test , botany , genomic dna , gene , biochemistry , genetics , enzyme , anatomy
An oval- to rod-shaped, motile, Gram-stain-negative, oxidase-positive, catalase-negative, pink-coloured phototrophic bacterium (designated as strain JA968 T ) was isolated from an estuary near Pata, Gujarat, India. Cells had an intracytoplasmic membrane architecture as lamellae and divided by budding. Strain JA968 T had bacteriochlorophyll- a and spirilloxanthin series carotenoids as photosynthetic pigments. The strain exhibited photolithoautotrophic, photoorganoheterotrophic and chemoorganoheterotrophic growth modes and required thiamine as a growth factor. Strain JA968 T had C 18 : 1 ω7 c /C 18 : 1 ω6 c as the predominant fatty acid with ubiquinone-10 (Q-10) and menaquinone-10 (MK-10) forming the quinone composition. The genomic DNA G+C content of the strain was 63.5 mol%. Pairwise comparison of 16S rRNA gene sequences showed that strain JA968 T was highly similar to Afifella marina DSM 2698 T (99.9 %) and Afifella pfennigii DSM 17143 T (98.4 %). The average nucleotide identity values were 92 % between strain JA968 T and A. marina DSM 2698 T , and 78 % between strain JA968 T and A. pfennigii DSM 17143 T . The digital DNA-DNA hybridization values between strain JA968 T and A. marina and A. pfennigii were 49 and 19 %, respectively. The genomic distinction was also supported by differences in phenotypic and chemotaxonomic characteristics. We propose that strain JA968 T represents a new species of the genus Afifella with the name Afifella aestuarii sp. nov. The type strain is JA968 T (=KCTC 15634 T =NBRC 113338 T ).