Open AccessMicromonospora deserti sp. nov., isolated from the Karakum Desert
Author(s) -
Hayrettin Saygın,
Hilal Ay,
Kıymet Güven,
Demet Çetin,
Nevzat Şahi̇n
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003752
Subject(s) - micromonospora , biology , 16s ribosomal rna , strain (injury) , actinomycetales , actinobacteria , peptidoglycan , phylogenetic tree , microbiology and biotechnology , botany , gene , streptomyces , bacteria , cell wall , genetics , anatomy
An isolate, 13K206 T , with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliation of the strain. The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso - and 3-OH-A 2 pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to ' Micromonospora spongicola ' S3-1 T , Micromonospora nigra DSM 43818 T and Micromonospora yasonensis DS3186 T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively. Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp. nov. is proposed. The type strain is 13K206 T (=JCM 32583 T =DSM 107532 T ). The DNA G+C content of the type strain is 72.4 mol%.