Open AccessPseudomonas bubulae sp. nov., isolated from beef
Author(s) -
Sonja Lick,
Lothar Kröckel,
Daniel Wibberg,
Annika Winkler,
Jochen Blom,
Alexander Goesmann,
Jörn Kalinowski
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003751
Subject(s) - biology , rpob , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , phosphatidylglycerol , pseudomonas , strain (injury) , gene , bacteria , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
Two Pseudomonas isolates derived independently from raw refrigerated processing meat of bovine origin intended for the manufacture of Bologna-type cooked sausage could be distinguished from other known species in subsequent phylogenetic analyses. Comparison of the complete rpo B gene sequences in combination with nearly complete 16S rRNA gene sequences revealed a separate branch within the Pseudomonas fragi group. In further analyses, comprising phenotypic and chemotaxonomic characterization as well as average nucleotide identity (ANI) values obtained from the draft genome assemblies, the two isolates could be distinguished from all so far published closely related species. The closest relative was P. fragi DSM 3456 T with ANI values of about 90.2 %. Other close Pseudomonas neighbours were P. psychrophila DSM 17535 T (86.5 %), P. deceptionensis DSM 26521 T (86.4 %), P. versuta DSM 101070 T (83.8 %), P. taetrolens DSM 21104 T (83.2 %), P. weihenstephanensis DSM 29166 T (82.3 %), P. helleri DSM 29165 T (82.7 %) and P. lundensis DSM 6252 T (81.9 %). The G+C contents of isolates TH39 T and TH26 were both 58.2 mol%. The major cellular lipids of strain TH39 T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major quinone was Q9 with small amounts of Q8. Based on these data, the isolates TH39 T and TH26 (=DSM 107389=LMG 30831) represent a novel species within the genus Pseudomonas , for which the name Pseudomonas bubulae sp. nov. is proposed. The type strain is TH39 T (=DSM 107390 T =LMG 30830 T ).