Open AccessSaliniradius amylolyticus gen. nov., sp. nov., isolated from solar saltern sediment
Author(s) -
Heeyoung Kang,
Inseong Cha,
Haneul Kim,
Kiseong Joh
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003748
Subject(s) - biology , taxonomy (biology) , microbiology and biotechnology , botany
A novel non-pigmented, Gram-stain-negative, motile by means of a polar flagellum, aerobic and rod-shaped bacterium, designated HMF8227 T , was isolated from solar saltern sediment sampled at Shinan, Republic of Korea. The isolate was able to grow at 15-42 °C (optimum, 37 °C), at pH 6-8 (pH 7) and with 0.5-12 % NaCl (2-5 %). Strain HMF8227 T was positive for hydrolysis of starch and dextrin. 16S rRNA gene sequence analysis revealed that strain HMF8227 T was affiliated with the family Alteromonadaceae , sharing the highest sequence similarities to the genera Salinimonas (93.0-94.4 %), Aestuariibacter (92.0-94.2 %) , Alteromonas (92.0-93.6 %) and Lacimicrobium (93.6 %). In the phylogenetic trees, strain HMF8227 T formed an independent clade with Lacimicrobium alkaliphilum X13M-12 T . The major fatty acids were C 16 : 0 , summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ). The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified glycolipids. The DNA G+C content of the genomic DNA was 52.1 mol%. On the basis of the polyphasic characterizations, strain HMF8227 T represents a novel species and genus within the family Alteromonadaceae , for which the name Saliniradius amylolyticus gen. nov., sp. nov. is proposed, with the type strain being HMF8227 T (=KCTC 62462 T =NBRC 113230 T ).