Open AccessKomagataeibacter diospyri sp. nov., a novel species of thermotolerant bacterial nanocellulose-producing bacterium
Author(s) -
Kallayanee Naloka,
Pattaraporn Yukphan,
Minenosuke Matsutani,
Kazunobu Matsushita,
Gunjana Theeragool
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003745
Subject(s) - biology , nanocellulose , bacteria , microbiology and biotechnology , cellulose , biochemistry , genetics
Thermotolerant bacterial nanocellulose-producing strains, designated MSKU 9 T and MSKU 15, were isolated from persimmon and sapodilla fruits, respectively. These strains were aerobic, Gram-stain-negative, had rod-shaped cells, were non-motile and formed white-cream colonies. Phylogeny based on the 16S rRNA gene sequences revealed that MSKU 9 T and MSKU 15 represented members of the genus Komagataeibacter and formed a monophyletic branch with K. swingsii JCM 17123 T and K. europaeus DSM 6160 T . The genomic analysis revealed that overall genomic relatedness index values of MSKU 9 T with K. swingsii JCM 17123 T and K. europaeus DSM 6160 T were ~90 % average nucleotide identity (ANI) and ≤58.2 % digital DNA-DNA hybridization (dDDH), respectively. MSKU 9 T and MSKU 15 can be differentiated from the closely related K. swingsii JCM 17123 T by their growth on 30 % d-glucose and ability to utilize and to form acid from raffinose and sucrose as carbon sources, and from K. europaeus DSM 6160 T by their ability to grow without acetic acid. The genomic DNA G+C contents of MSKU 9 T and MSKU 15 were 60.4 and 60.2 mol%, respectively. The major fatty acids of MSKU 9 T and MSKU 15 were summed feature 8 (C 18 : 1 ω7c and/or C 18 : 1 ω6c). The respiratory quinone was determined to be Q10. On the basis of the results of the polyphasic taxonomic analysis, MSKU 9 T (=TBRC 9844 T =NBRC 113802 T ) represents a novel species of the genus Komagataeibacter , for which the name Komagataeibacter diospyri sp. nov. is proposed.