Open AccessStreptomyces bauhiniae sp. nov., isolated from tree bark of Bauhinia variegata Linn. in Thailand
Author(s) -
Pawina Kanchanasin,
Masahiro Yuki,
Takuji Kudo,
Moriya Ohkuma,
Nattakorn Kuncharoen,
Wongsakorn Phongsopitanun,
Somboon Tanasupawat
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003743
Subject(s) - biology , streptomyces , botany , strain (injury) , muramic acid , microbiology and biotechnology , bacteria , peptidoglycan , cell wall , genetics , anatomy
A novel actinomycete strain, Bv016 T , belonging to the genus Streptomyces , was isolated from the bark of tree, Bauhinia variegata Linn., collected in Thailand. The taxonomic position of the strain was characterized by using a polyphasic approach. ll-Diaminopimelic acid, glucose, mannose, ribose and galactose were detected in its whole-cell hydrolysates. The N -acyl type of muramic acid was acetyl. The strain contained anteiso-C 15 : 0 , iso-C 16 : 0 , iso-C 15 : 0 and iso-C 14 : 0 as the major fatty acids and MK-9(H 8 ), MK-9(H 6 ) and MK-9(H 4 ) as the major menaquinones. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The strain was closely related to Streptomyces griseoluteus JCM 4041 T (98.4 %), Streptomyces seoulensis JCM 10116 T (98.4 %) and Streptomyces recifensis JCM 4408 T (98.2 %). The draft genome of Bv016 T was 6.74 Mb with 5949 coding sequences with an average G+C content of 71.7 mol%. The ANIb and ANIm values of strain Bv016 T were 94.1 and 95.2 %, respectively, and the digital DNA-DNA hybridization value was 60.6 % in comparison with the draft genome of S. griseoluteus JCM 4765 T . The results of the taxonomic analysis suggested that strain Bv016 T represented a novel species of the genus Streptomyces for which the name Streptomyces bauhiniae sp. nov. is proposed. The type strain is Bv016 T (=JCM 33208 T =TISTR 2645 T ).