Open AccessPsychrobacter pygoscelis sp. nov. isolated from the penguin Pygoscelis papua
Author(s) -
Peter Kämpfer,
Stefanie P. Glaeser,
Rute Irgang,
Guillermo Fernández-Negrete,
Matías PobleteMorales,
Derie E. Fuentes,
Marcelo Cortez San Martín,
Rubén AvendañoHerrera
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003739
Subject(s) - pygoscelis , biology , zoology , microbiology and biotechnology , ecology , foraging
One slightly beige-white pigmented, Gram-stain-negative, rod-shaped bacterium, strain I-STPP5b T , was isolated from the trachea of a Gentoo penguin chick individual ( Pygoscelin papua ) investigated in Fildes Bay, Chilean Antarctic (62° 12' S, 58° 57' W). I-STPP5b T consists of a 3.4 Mb chromosome with a DNA G+C content of 44.4 mol%. Of the 3056 predicted genes, 1206 were annotated as hypothetical proteins and 51 were tRNAs. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared a 16S rRNA gene sequence identity to the type strains of Psychrobacter phenylpyruvicus (98.8 %), Psychrobacter arenosus and Psychrobacter pasteurii (both 98.3 %), Psychrobacter piechaudii (98.2 %) and Psychrobacter sanguinis (98.1 %), but 16S rRNA gene sequence similarities to all other Psychrobacter species were ≤98.0 %. Partial gyrB nucleotide and amino acid sequence similarities among strain STPP5b T and the next related type strains were all below 81.8 and 92.9%, respectively. DNA-DNA hybridisation (DDH) with P. phenylpyruvicus LMG 5372 T , P. arenosus DSM 15389 T and P. sanguinis DSM 23635 T also showed low values (all below 30 %). The main cellular fatty acids of the strain were C 18 : 1 ω9c and C 16 : 1 ω7c and/or C 16 : 1 ω6c. Based on phylogenetic, chemotaxonomic, genomic and phenotypic analyses we propose a new species of the genus Psychrobacter, with the name Psychrobacter pygoscelis sp. nov. and strain I-STPP5b T (=CIP 111410 T = CCM 8799 T =LMG 30301 T ) as type strain.