Nocardioides guangzhouensis sp. nov., an actinobacterium isolated from soil

A Gram-positive, aerobic, non-spore-forming, non-pigmented and non-motile actinobacterial strain was isolated from a soil sample collected in Guangzhou, China. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 130 T is most closely related to the type strain Nocardioides iriomotensis NBRC 105384 T , with a sequence similarity of 97.69 %. The isolate was distinguished from this phylogenetically related type strain by DNA-DNA hybridization (33.3 %), digital DNA-DNA hybridization (21.2 %), average nucleotide identity (75.7 %) and by a range of physiological and biochemical characteristics. Strain 130 T contained MK-8(H 4 ) and MK-7 as the major menaquinones, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol as the main polar lipids, and iso-C 16 : 0 , C 17 : 1 ω8 c , anteiso-C 17 : 0 , C 18 : 1 ω9 c , C 17 : 0 and iso-C 14 : 0 as the major cellular fatty acids. The novel strain grew at 20-36 °C, at pH 6.0-8.0 and in the presence of 0-6 % (w/v) NaCl. The genomic DNA G+C content was 72.9 mol%. The genome contained 4817 putative protein-coding sequences, and 45 tRNA and three rRNA genes. Phylogenomic analysis confirmed that strain 130 T belongs to the genus Nocardioides and distinguished it from recognized Nocardioides species with available genomes. Based on these polyphasic taxonomic data, strain 130 T represents a new species of the genus Nocardioides , for which the name Nocardioidesguangzhouensis sp. nov. is proposed. The type strain is 130 T (=CICC 24668 T =JCM 33269 T ).