Open AccessMicrobispora fusca sp. nov., a novel actinomycete isolated from the ear of wheat (Triticum aestivum L.)
Author(s) -
Junwei Zhao,
Bing Yu,
Chuanyu Han,
Peng Cao,
Zhiyin Yu,
Hanxun Ju,
Wensheng Xiang,
Xiangjing Wang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003725
Subject(s) - biology , 16s ribosomal rna , botany , actinomycetales , strain (injury) , gene sequence , actinobacteria , microbiology and biotechnology , gene , streptomyces , genetics , bacteria , anatomy
A novel actinomycete, designated strain NEAU-HEGS1-5 T , was isolated from the ear of wheat ( Triticum aestivum L.) and characterized using a polyphasic approach. The morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus Microbispora . The results of 16S rRNA gene sequence analysis showed that the isolate was most closely related to Microbispora bryophytorum NEAU-TX2-2 T (99.3 %), Microbispora camponoti 2C-HV3 T (99.2 %), Microbispora amethystogenes JCM 3021 T (99.1 %) and Microbispora rosea subsp. rosea JCM 3006 T (98.5 %). However, two tree-making algorithms supported the position that strain NEAU-HEGS1-5 T formed a distinct clade with M. bryophytorum NEAU-TX2-2 T , M. camponoti 2C-HV3 T and M. rosea subsp . rosea JCM 3006 T . Furthermore, multilocus sequence analysis based on the 16S- gyr B - rpo B genes and a combination of DNA-DNA hybridization results and some physiological and biochemical properties demonstrated that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-HEGS1-5 T should be classified as representative of a novel species of the genus Microbispora , for which the name Microbispora fusca sp. nov. is proposed. The type strain is NEAU-HEGS1-5 T (=CCTCC AA 2019030 T =DSM 104648 T ).