Open AccessFlavobacterium ustbae sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia
Author(s) -
Yang Liu,
Ho Le Han,
Yuanyuan Zou,
SongGun Kim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003717
Subject(s) - biology , 16s ribosomal rna , rhizosphere , flavobacterium , spermidine , strain (injury) , phylogenetic tree , microbiology and biotechnology , botany , bacteroidetes , bacteria , genomic dna , gene , genetics , biochemistry , pseudomonas , anatomy , enzyme
A yellow-pigmented bacterial strain, designated T13 T , was isolated from the rhizosphere soil of Alhagi sparsifolia collected from Xinjiang, PR China. Cells were rod-shaped, Gram-stain-negative, aerobic and gliding. Strain T13 T grew optimally at 25-30 °C and pH 7.0-8.0 with a NaCl tolerance of 0-2 % on Reasoner's 2A agar. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain T13 T belonged to the genus Flavobacterium within the family Flavobacteriaceae and was closely related to Flavobacterium nitrogenifigens KCTC 42884 T with a similarity value of 97.4 %. The major polar lipid was phosphatidylethanolamine; the only respiratory quinone was MK-6, and the polyamine profile contained sym -homospermidine as the major polyamine and a trace amount of spermidine. The major fatty acids were iso-C 15 : 0 , C16 : 1 ω7 c and summed feature 3 (comprising C 16 : 1 ω7 c or C 16 : 1 ω6 c ). The G+C content of the genomic DNA was 34.1 mol%. It is concluded from the phenotypic and genotypic data that strain T13 T represents a novel species of the genus Flavobacterium , for which the name Flavobacteriumustbae sp. nov. with the type strain T13 T (=KCTC 62874 T =ACCC 60126 T ) is proposed.