Open AccessBacillus cabrialesii sp. nov., an endophytic plant growth promoting bacterium isolated from wheat (Triticum turgidum subsp. durum) in the Yaqui Valley, Mexico
Author(s) -
Sergio de los Santos-Villalobos,
Rosa I Robles,
Fannie Isela Parra-Cota,
John Larsen,
Paulina Lozano,
James M. Tiedje
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003711
Subject(s) - biology , 16s ribosomal rna , bacillus subtilis , bacteria , bacillus (shape) , strain (injury) , botany , microbiology and biotechnology , food science , genetics , anatomy
Strain TE3 T , an endophytic plant growth promoting bacterium, was isolated from wheat ( Triticumturgidum subsp. durum ) sampled in the Yaqui Valley, Mexico. Biochemical, phenotypic and genotypic approaches were used to clarify the taxonomic affiliation of this strain. Based on analysis of its full-length 16S rRNA gene, strain TE3 T was assigned to the genus Bacillus (similarity ≥98.7 %). This finding was supported by morphological and metabolic characteristics, such as rod shape, strictly aerobic metabolism, spore formation, Gram-positive staining, catalase-positive activity, reduction of nitrate to nitrite, starch and casein hydrolysis, growth in presence of lysozyme and 2 % NaCl, citrate utilization, growth pH from 6.0 to 8.0, and acid and indole production from glucose and tryptophan, respectively. The whole-genome phylogenetic relationship showed that TE3 T formed an individual clade with Bacillus tequilensis KCTC 13622 T , distant from that generated by all Bacillus subtilis subspecies. The maximum values for average nucleotide identity and in silico DNA-DNA hybridization were 93.85 and 54.30 %, respectively, related to Bacillus subtilis subsp. inaquosorum KCTC 13429 T . Analysis of its fatty acid content showed the ability of strain TE3 T to bio-synthetize fatty acids that are not present in closely related Bacillus species, such as C 12 : 0 , C 12 : 0 2OH, C 12 : 0 3OH, C 17 : 0 , iso-C 17 : 0 3OH and C 18 : 1 ω9 c . These results provide evidence that strain TE3 T is a novel species of the genus Bacillus , for which the name Bacilluscabrialesii sp. nov. is proposed. The type strain of Bacilluscabrialesii is TE3 T (CM-CNRG TB54 T =CCStamb A1 T ).