Open AccessFlavobacterium macacae sp. nov., isolated from Macaca mulatta faeces
Author(s) -
Gui-Ding Li,
Yi Jiang,
You-Long Li,
Xiu Chen,
Kun Zhang,
Rui Shen,
Li Han,
ChengLin Jiang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003708
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , flavobacterium , strain (injury) , microbiology and biotechnology , bacteria , phylogenetics , flavobacteriaceae , ribosomal rna , botany , gene , bacteroidetes , genetics , pseudomonas , anatomy
A yellow, Gram-stain-negative, aerobic, non-gliding, non-spore-forming, rod-shaped strain, designated YIM 102600 T , was isolated from the faeces of Macaca mulatta dwelling in the Yunnan Wild Animal Park, Yunnan Province, South-West PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 102600 T was a member of the genus Flavobacterium , and closely related to Flavobacterium qiangtangense F3 T (96.9 % similarity) and Flavobacterium noncentrifugens R-HLS-17 T (96.0 % similarity). Phylogenetic trees showed that strain YIM 102600 T formed a clade with F. qiangtangense F3 T and F. noncentrifugens R-HLS-17 T . Growth occurred at 4-30 °C (optimum, 28 °C), pH 7.0-8.0 (pH 7.5) and NaCl concentration 0-2 % (w/v; 0-1 %, w/v). The major fatty acids were iso-C 15:0 and summed feature 3 (comprising C 16:1 ω 7 c and/or C 16:1 ω 6 c ). The predominant polar lipid was phosphatidylethanolamine and the sole respiratory quinone was menaquinone-6. The DNA G+C content was 36.4 mol%. The calculated digital DNA-DNA hybridization values between strain YIM 102600 T and other species of Flavobacterium ranged from 70.0 to 75.0 % and average nucleotide identity values were in a range between 13.7 to 23.5 %. Based above the consensus of phenotypic and phylogenetic analyses as well as whole genome comparisons, strain YIM 102600 T (=KCTC 52099 T =CCTCC AB 201632 T ) is proposed to represent type strain of a novel species, Flavobacterium macacae sp. nov.