Open AccessFlavobacterium cellulosilyticum sp. nov., a novel psychrophilic bacterium isolated from Arctic soil
Author(s) -
Dhiraj Kumar Chaudhary,
Khulan Altankhuu,
Donguk Kim,
Jaisoo Kim
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003707
Subject(s) - psychrophile , 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , flavobacterium , bacteria , microbiology and biotechnology , soil microbiology , botany , gene , genetics , pseudomonas , anatomy
A novel psychrophilic, light-yellow-coloured, aerobic, Gram-stain-negative, rod-shaped, non-motile bacterium, designated strain AR-3-4 T was isolated from a sample of Arctic soil. Strain AR-3-4 T grew at 0-25 °C, pH 6.0-9.0 and 0-1.0 % (w/v) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain AR-3-4 T belonged to the genus Flavobacterium , with nearest phylogenetic neighbour being Flavobacterium fluvii H7 T (97.5 % sequence similarity). The strain comprised phosphatidylethanolamine as the main polar lipid, MK-6 as predominant respiratory quinone, and summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), anteiso-C 15 : 0 and iso-C 15 : 0 as the major fatty acids. The average nucleotide identity and in silico DNA-DNA hybridization values between strain AR-3-4 T and closest members were below the threshold values of 95 % and 70 %, respectively. The DNA G+C content was 33.0 mol%. Based on the polyphasic taxonomic data, the novel species Flavobacterium cellulosilyticum sp. nov. is proposed with the type strain AR-3-4 T (=KEMB 9005-740 T =KACC 21171 T =NBRC 113787 T ).