Rhodoferax bucti sp. nov., isolated from fresh water

A Gram-reaction-negative, peach-brown-pigmented, slightly curved-rod-shaped, aerobic, non-motile bacterium, designated GSA243-2 T , was isolated from fresh water samples collected from the Chishui River flowing through Maotai, Guizhou, south-west PR China. Phenotypic, chemotaxonomic and genomic traits were investigated. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Rhodoferax . The closest phylogenetic relative was Rhodoferax saidenbachensis ATCC BAA-1852 T (98.35 %). The major fatty acids were C 16: 0 and C 16 : 1 ω6 c and/or C 16 : 1 ω7 c . The major respiratory quinone was ubiquinone Q-8 and the major polar lipid was phosphatidylethanolamine. Genome sequencing revealed a genome size of 3.67 Mbp and a G+C content of 61.17 mol%. Pairwise-determined whole genome average nucleotide identity values and digital DNA-DNA hybridization values suggested that strain GSA243-2 T represents a new species, for which we propose the name Rhodoferaxbucti sp. nov. with the type strain GSA243-2 T (=CGMCC 1.16288 T =KCTC 62564 T ).