Open AccessRhodoferax bucti sp. nov., isolated from fresh water
Author(s) -
De Zhou,
Xu Tan,
Wei Zhang,
HanYi Chen,
Qiu-Ming Fan,
Xianlin He,
Jie Lv
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003700
Subject(s) - biology , phylogenetic tree , 16s ribosomal rna , genomic dna , strain (injury) , genome size , genome , ribosomal rna , bacteria , botany , gene , mitochondrial dna , genetics , anatomy
A Gram-reaction-negative, peach-brown-pigmented, slightly curved-rod-shaped, aerobic, non-motile bacterium, designated GSA243-2 T , was isolated from fresh water samples collected from the Chishui River flowing through Maotai, Guizhou, south-west PR China. Phenotypic, chemotaxonomic and genomic traits were investigated. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Rhodoferax . The closest phylogenetic relative was Rhodoferax saidenbachensis ATCC BAA-1852 T (98.35 %). The major fatty acids were C 16: 0 and C 16 : 1 ω6 c and/or C 16 : 1 ω7 c . The major respiratory quinone was ubiquinone Q-8 and the major polar lipid was phosphatidylethanolamine. Genome sequencing revealed a genome size of 3.67 Mbp and a G+C content of 61.17 mol%. Pairwise-determined whole genome average nucleotide identity values and digital DNA-DNA hybridization values suggested that strain GSA243-2 T represents a new species, for which we propose the name Rhodoferaxbucti sp. nov. with the type strain GSA243-2 T (=CGMCC 1.16288 T =KCTC 62564 T ).