Open AccessLeucothrix sargassi sp. nov., isolated from a marine alga [Sargassum natans (L.) Gaillon]
Author(s) -
Tingwei Liu,
Yao Zhang,
Xuan Zhang,
Lixin Zhou,
Chunyu Meng,
Chenyan Zhou,
Changqin Jing,
Wuling Zhu,
Yan Wang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003694
Subject(s) - biology , 16s ribosomal rna , phosphatidylglycerol , phylogenetic tree , strain (injury) , microbiology and biotechnology , genomic dna , botany , phospholipid , biochemistry , gene , phosphatidylcholine , anatomy , membrane
A novel bacterial strain, C3212 T , was isolated from a marine alga collected from the sea shore of Yantai, China. The strain was Gram-stain-negative, rod-shaped, aerobic, non-motile, and oxidase- and catalase-positive. Growth was observed at 8-37 °C (optimum, 28 °C), at pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1.0-7.0 % (w/v) NaCl (optimum, 4.0 %). The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids of strain C3212 T consisted of diphosphatidylglycerol (cardiolipin), phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified polar lipid. The major fatty acids were C 16 : 1 ω 6c and/or C 16 : 1 ω 7c , and C 18 : 1 ω 6c and/or C 18 : 1 ω 7c . The DNA G+C content of strain C3212 T was 44.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Leucothrix pacifica XH122 T , Leucothrix arctica IMCC 9719 T and Leucothrix mucor DSM 2157 T with similarities of 98.0, 97.5 and 94.3 %, respectively. Estimated DNA-DNA hybridization values were 14.2, 20.7 and 13.9 % between strain C3212 T and L. pacifica XH122 T , L. arctica IMCC 9719 T and L. mucor DSM 2157 T , respectively. Phenotypic, phylogenetic and genomic analyses revealed that strain C3212 T represents a novel species of the genus Leucothrix , for which the name Leucothrix sargassi sp. nov. is proposed. The type strain is C3212 T (=MCCC 1K03600 T =KCTC 72121 T ).