Rhodoligotrophos defluvii sp. nov., isolated from activated sludge

A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped and aerobic bacterial strain (designated lm1 T ) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong province, PR China). Strain lm1 T grew between 15 and 45 °C (optimum, 40 °C). The pH range for growth was at pH 4.0-10.0 (optimum growth at pH 8.0). The range of NaCl concentration for the growth of strain lm1 T was 0-4.0 % (w/v), with optimum growth at 1.0-2.0 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain lm1 T belonged to the genus Rhodoligotrophos and was closely related to Rhodoligotrophos appendicifer JCM 16873 T (96.7 % 16S rRNA gene sequence similarity) and Rhodoligotrophosjinshengii CCTCC AB2013083 T (96.2 %). The average nucleotide identity value and the digital DNA-DNA hybridization value between strain lm1 T and R. appendicifer JCM 16873 T were 73.4 and 14.3 %, respectively. The major cellular fatty acids of strain lm1 T were C 19 : 0 cyclo ω8 c , C 18 : 1 ω7 c and C 16 : 0 . Strain lm1 T contained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and five unknown lipids. The G+C content of the genomic DNA was 64.4 mol%. Based on polyphasic taxonomic data, strain lm1 T could be classified as a representative of a novel species of the genus Rhodoligotrophos , for which the name Rhodoligotrophosdefluvii sp. nov. is proposed. The type strain is lm1 T (=CCTCC AB2019071 T =KCTC 72156 T ).