Open AccessPaenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil
Author(s) -
Silvina Ghio,
Diego Herman Sauka,
Alejandro E. Ferrari,
Florencia E Piccini,
Ornella Mailén Ontañon,
Eleonora Campos
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003686
Subject(s) - biology , 16s ribosomal rna , paenibacillus , housekeeping gene , microbiology and biotechnology , rpob , bacteria , phylogenetic tree , strain (injury) , sequence analysis , soil microbiology , botany , gene , genetics , gene expression , anatomy
A xylanolytic bacterial strain, named A59 T , was isolated from a forest soil consortium in southern Argentina. Strain A59 T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its optimal growth conditions are 30 °C (range, 28-37 °C), pH 7 (range, pH 5-10) and it tolerates up to 7 % of NaCl (range, 2-7 %). Chemotaxonomic analysis revealed that strain A59 T possesses meso -diaminopimelic acid in the cell wall. It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C 15 : 0 (35.1 %), with a moderate amount of C 16 : 0 (6.9 %). According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as Paenibacillus taichungensis BCRC 17757 T (99.7 % nucleotide sequence identity) and Paenibacillus pabuli NBRC 13638 T (99.1 %) and is closely related to Paenibacillus tundrae A10b T (98.8 %). However, phylogenetic studies based on the housekeeping gyrB gene placed A59 T in a separate branch from all other related type strains. Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA-DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species. The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing. On the basis of these results, A59 T represents a new species of the genus Paenibacillus , and we propose the name Paenibacillusxylanivorans sp. nov. The type strain is A59 T (=DSM 107920 T =NCIMB 15123 T ).