Open AccessMuricauda hymeniacidonis sp. nov., isolated from sponge of Hymeniacidon sinapium
Author(s) -
Jin-Sook Park
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003683
Subject(s) - biology , 16s ribosomal rna , sponge , bacteria , strain (injury) , genomic dna , microbiology and biotechnology , bacteroidetes , genus , botany , gene , biochemistry , genetics , anatomy
A Gram-stain-negative, rod-shaped, non-motile, aerobic, orange-pigmented, bacterium (designated 176CP4-71 T ) was isolated from a sponge sample of Hymeniacidonsinapium from Cheongpo beach, Taean district, in the Republic of Korea. On the basis of 16S rRNA gene sequencing, strain 176CP4-71 T clustered with species of the genus Muricauda and appeared to be closely related to Muricaudapacifica LMG 26637 T (95.4 %), Muricaudaantarctica JCM 18450 T (94.7 %), Muricaudataeanensis KACC 16195 T (94.7 %) and Muricaudalutaonensis KCTC 22339 T (94.2 %). The average nucleotide identity values between strain 176CP4-71 T and the closely related strains were 70.7-73.7 %, indicating that 176CP4-71 T is a novel species of the genus Muricauda . Growth occurs at 10-35 °C on marine agar medium in the presence of 1-8 % NaCl (w/v) and at pH 6.0-8.5. The DNA G+C content of the genomic DNA was 41.6 mol %, and menaquinone with six isoprene units (MK-6) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C 15 : 0 iso, C 15 : 1 iso G, C 17 : 0 iso 3OH and C 16:1 ω6 c and/or C 16:1 ω7 c (summed feature 3). The polar lipids consisted of phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified phospholipid and unidentified lipids. Physiological and biochemical characteristics indicated that strain 176CP4-71 T represents a novel species of the genus Muricauda , for which the name Muricauda hymeniacidonis sp. nov. is proposed. The type strain is 176CP4-71 T (=KACC 19889 T =LMG 31033 T ).