Thalassotalea mangrovi sp. nov., a bacterium isolated from marine mangrove sediment

A novel Gram-stain-negative, strictly aerobic bacterium that has a rod-like shape with a single polar flagellum in the exponential phase of growth and a spherical or ovoid shape without a flagellum in the stationary phase was isolated from a mangrove wetland sediment sample collected at Beilun Estuary National Nature Reserve, Guangxi Province, PR China and designated strain ZS-4 T . This strain grew optimally at pH 6.0-8.0, at a temperature of 37 °C and in the presence of 3-4 % (w/v) NaCl. Its polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid and two uncharacterized lipids. Ubiquinone 8 (Q-8) was the sole respiratory quinone and the cellular fatty acids were dominated by C 17 : 1 ω8 c and C 16 : 0 . A phylogenetic analysis based on the 16S rRNA gene sequence showed that strain ZS-4 T exhibited its highest similarities to the type strains Thalassotalea litorea HMF4135 T (97.8 %) and Thalassotalea ponticola GJSW-36 T (95.9 %). A whole genome-level comparison of strain ZS-4 T with T. litorea MCCC 1K03283 T revealed an average nucleotide identity value of 75.6 % and a calculated DNA-DNA hybridization value of 19.6 %. In addition, the genomic DNA G+C content of strain ZS-4 T was 45.9 mol%. Thus, based on analyses of its morphology, physiology, fatty acid composition and 16S rRNA gene sequence, strain ZS-4 T should be considered a novel species of the genus Thalassotalea , with the proposed name Thalassotaleamangrovi sp. nov. The type strain is ZS-4 T (=KCTC 72399 T =MCCC 1K03630 T ).