Open AccessNocardioides yefusunii sp. nov., isolated from Equus kiang (Tibetan wild ass) faeces
Author(s) -
YuYuan Huang,
Xiaoxia Wang,
Jing Yang,
Shan Lu,
XinHe Lai,
Dong Jin,
Ji Pu,
Ying Huang,
Zhihong Ren,
Wentao Zhu,
Jiajia Meng,
Kui Dong,
Yajun Ge,
Jia-Rong Zhou,
Zhilun Gui,
Sihui Zhang,
Wenjing Lei,
Hao Liang,
Jianguo Xu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003674
Subject(s) - biology , diamino acid , 16s ribosomal rna , microbiology and biotechnology , strain (injury) , actinomycetales , bacteria , genetics , streptomyces , gene sequence , anatomy
Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056 T and HY057, were isolated from the faeces of Equus kiang (the largest of the wild asses) collected at different regions from the Qinghai-Tibetan Plateau of PR China. Phylogenetic analyses indicated that strains HY056 T and HY057 belong to the genus Nocardioides by sharing a similarity ranging from 96.3 to 97.0 % in the 16S rRNA gene sequence and forming a distinct cluster with Nocardioides daphniae JCM 16608 T , Nocardioides houyundeii 78 T , Nocardioides solisilvae Ka25 T and Nocardioides gilvus XZ17 T . The digital DNA-DNA hybridization value of strain HY056 T was 96.9 % with strain HY057, but less than 30.0 % with the above four closest relatives. MK-8(H 4 ) was the predominant (91.6 %) respiratory quinone. The cell wall contained ll-2,6-diaminopimelic acid as the diamino acid of the peptidoglycan. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phospholipid. The predominant fatty acids (>10.0 %) were C 18 : 1 ω9 c and iso -C 16:0. The DNA G+C contents of strains HY056 T and HY057 were 68.9 and 69.1 mol%, respectively. β -Glucosidase expression was positive, and acid was produced from d-fructose. Strain HY056 T (=CGMCC 4.7563 T =JCM 33399 T ) is assigned as the type strain of a novel species within the genus Nocardioides , for which the name Nocardioidesyefusunii sp. nov is proposed.