Open AccessPaenibacillus ottowii sp. nov. isolated from a fermentation system processing bovine manure
Author(s) -
Luis F Velazquez,
Shashi Rajbanshi,
Shaohua Guan,
Maud Hinchee,
Allana Welsh
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003672
Subject(s) - biology , 16s ribosomal rna , rpob , peptidoglycan , diamino acid , microbiology and biotechnology , fermentation , strain (injury) , glycolipid , biochemistry , food science , botany , gene , gene sequence , anatomy
Strain MS2379 T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas. Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379 T was most closely related to Paenibacillus polymyxa (DSM 36 T ) , P. jamilae (DSM 13815 T ) , and P. peoriae (DSM 8320 T ) , yet DNA–DNA relatedness through DNA–DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively. Rod-shaped cells of strain MS2379 T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores. The peptidoglycan contains meso -diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C 15 : 0 (61.9 %) and anteiso-C 17 : 0 (11.6 %), confirming that strain MS2379 T has diagnostic features of other Paenibacillus species. The G+C content of MS2379 T is 45.9 mol%. Fermentation of glucose yields acid and gas end-products. The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine. The growth range of MS2379 T was observed from 10–45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6–10 and up to 3 % NaCl. Unlike the most closely related Paenibacillus species, strain MS2379 T was negative in the Voges-Proskauer reaction. Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379 T . Thus, strain MS2379 T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp. nov. is proposed with the type strain MS2379 T (=DSM 107750 T =ATCC TSD-165 T ).