Open AccessPontibacter arcticus sp. nov., isolated from rhizosphere soil of Saxifraga oppositifolia
Author(s) -
Zhenwei Liu,
Jing Zhang,
Yong Yu,
Huirong Li,
ZongJun Du
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003668
Subject(s) - 16s ribosomal rna , biology , rhizosphere , phylogenetic tree , strain (injury) , botany , genus , genomic dna , bacteria , gene , genetics , anatomy
A Gram-stain-negative, rod-shaped, red-coloured and aerobic bacterium, designated 2b14 T , was isolated from rhizosphere soil of Saxifraga oppositifolia sampled at the Chinese Arctic Yellow River Station in Norway. Optimal growth occurred at 28 °C (range, 4-37 °C) and pH 7.0-7.5 (pH 6.5-8.5). The strain could tolerate up to 2.5 % (w/v) NaCl concentration. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 2b14 T had the highest similarity value of 96.0 % to Pontibacter diazotrophicus CCTCC AB 2013049 T . The major fatty acids were summed feature 4 (anteiso-C 17 : 1 B and/or iso-C 17 : 1 I), iso-C 15 : 0 and iso-C 17 : 0 3-OH. The major polar lipid was found to be phosphatidylethanolamine. The genomic DNA G+C content of strain 2b14 T was 45.5 mol%. The sole respiratory quinone was MK-7. The combined results of physiological, genotypic, phylogenetic and chemotaxonomic analyses showed that strain 2b14 T represents a novel species within the genus Pontibacter , for which the name Pontibacter arcticus sp. nov. is proposed. The type strain is 2b14 T (=KCTC 62596 T =MCCC 1H00304 T ).