Open AccessApibacter muscae sp. nov., a novel bacterial species isolated from house flies
Author(s) -
Rahel Park,
Maria C. Dzialo,
Donat Nsabimana,
Bart Lievens,
Kevin J. Verstrepen
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003667
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , musca , strain (injury) , bacteria , microbiology and biotechnology , phylogenetics , genome , taxonomy (biology) , ribosomal rna , muscidae , bacterial strain , gene , zoology , botany , genetics , anatomy , larva
We describe the isolation and characterization of three bacterial isolates from the common house fly, Musca domestica , caught in Londerzeel, Belgium and Huye District, Rwanda. Although isolated from distinct geographical locations, the strains show >99 % identical 16S rRNA gene sequences and are <95 % identical to type strains of Apibacter species. Whole-genome sequences were obtained for all three strains. The genomes are 2.4-2.5 Mb with a G+C content of ~30.3 mol%. Bacteriological and biochemical analysis of the strains demonstrate distinctly different characteristics compared to known Apibacter species. Particularly, the three strains investigated in this study can be distinguished from the known Apibacter species ( Apibacter mensalis and Apibacter adventoris ) through urease and β-glucosidase activities. Whole-cell fatty acid methyl ester analysis shows that the fatty acid composition of the novel strains is also unique. On the basis of phylogenetic, genotypic and phenotypic data, we propose to classify these isolates as representatives of a novel species of the genus Apibacter, Apibacter muscae sp. nov., in reference to its prevalence in house flies, with strain G8 T (=LMG 30898 T =DSM 107922 T ) as the type strain.