Open AccessLentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi)
Author(s) -
Auttaporn Booncharoen,
Wonnop Visessanguan,
Nattakorn Kuncharoen,
Supalurk Yiamsombut,
Pannita Santiyat,
Wuttichai Mhuantong,
Salisa Charoensri,
Pornchai Rojsitthisak,
Somboon Tanasupawat
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003658
Subject(s) - biology , shrimp , halophile , bacteria , microbiology and biotechnology , botany , ecology , genetics
A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9 T , was isolated from traditional salted shrimp paste ( Ka-pi ) produced in Samut Sakhon Province, Thailand. This strain grew optimally at 37-40 °C, pH 7.0 and in the presence of 8-16 % (w/v) NaCl. The 16S rRNA gene sequence similarity values between strain SSKP1-9 T and Lentibacillus juripiscarius TISTR 1535 T and Lentibacillus halophilus TISTR 1549 T were 98.7 and 97.2 %, respectively. Based on 16S rRNA gene sequence similarity, strain SSKP1-9 T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values. In addition, the whole-cell protein profile confirmed the novelty of the taxon. The genomic DNA G + C content was 44.6 mol%. The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso -diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 . The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9 T represents a novel species of Lentibacillus , for which the name Lentibacilluslipolyticus sp. nov. is proposed. The type strain is SSKP1-9 T (=JCM 32625 T =TISTR 2597 T ).