Open AccessEmticicia agri sp. nov., a novel member of the family Cytophagaceae
Author(s) -
Leonid N. Ten,
Weilan Li,
Aeri Ha,
Myung Kyum Kim,
Alejandro P. Rooney,
Hee–Young Jung
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003651
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , genomic dna , strain (injury) , gene , ribosomal rna , bacteroidetes , orange (colour) , microbiology and biotechnology , botany , genetics , food science , anatomy
A bacterial strain, 17J42-9 T , was isolated from a soil sample collected on Jeju Island, Republic of Korea. Cells were observed to be Gram-stain negative and rod-shaped. Colonies were observed to be orange in colour on R2A agar. Analysis of 16S rRNA gene sequences showed that high levels of 16S rRNA sequence similarity were shared between 17J42-9 T and Emticicia fontis IMCC1731 T (98.2 %), Emticicia ginsengisoli Gsoil 085 T (98.2 %) and Emticicia soli ZZ-4 T (97.8 %). Growth of strain 17J42-9 T was observed at 10-37 °C, pH 6.0-8.5 and in the presence of 0-0.5 % NaCl. The genomic G+C content was calculated to be 38.6 mol%. The predominant respiratory quinone of the isolate was found to be MK-7; the major fatty acids were identified as summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) (34.1 %), C 15 : 0 iso (23.4 %) and C 17 : 0 iso 3-OH (10.8 %). The major polar lipids were found to be phosphatidylethanolamine, two unidentified aminolipids and an unidentified lipid. The phenotypic and chemotaxonomic data support the affiliation of strain 17J42-9 T with the genus Emticicia . However, the DNA-DNA relatedness between the isolate and its closest phylogenetic neighbours was lower than 46 %. The results of 16S rRNA gene sequence similarity analysis, DNA-DNA hybridization analysis and the observed differentiating phenotypic properties from other closely related taxa clearly indicate that strain 17J42-9 T represents a novel species in the genus Emticicia , for which the name Emticiciaagri sp. nov. is proposed. The type strain is 17J42-9 T (=KCTC 62270 T =JCM 33056 T ).